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High-resolution Mass Spectrometry of Skin Mucus for Monitoring Physiological Impacts and Contaminant Biotransformation Products in Fathead Minnows Exposed to Wastewater Effluent

Metadata Updated: November 12, 2020

High-resolution mass spectrometry is advantageous for monitoring physiological impacts and contaminant biotransformation products in fish exposed to complex wastewater effluent. We evaluated this technique using skin mucus from male and female fathead minnows (Pimephales promelas) exposed to control water or treated wastewater effluent at 5%, 20%, and 100% levels for 21 d, using an onsite, flow-through system providing real-time exposure. Both sex-specific and non-sex-specific responses were observed in the mucus metabolome, the latter suggesting the induction of general compensatory pathways for xenobiotic exposures. Altogether, 85 statistically significant treatment-dependent metabolite changes were observed and 30 of those annotated with probable structures. The mummichog software package was used to elucidate impacted biochemical pathways and enhance metabolite annotation. Partial least squares regression models revealed relationships between the mucus metabolomes and upregulated hepatic mRNA transcripts reported previously for these same fish. These regression models suggest that mucus metabolomic changes reflected, in part, processes by which the fish biotransformed xenobiotics in the effluent. Further, we detected a phase II transformation product of bisphenol A in the skin mucus of male fish. Collectively, these findings demonstrate the utility of mucus as a minimally invasive matrix for simultaneously assessing exposures and effects of real-world mixtures of contaminants.

This dataset is associated with the following publication: Mosley, J., D. Ekman, J.E. Cavallin, D. Villeneuve, G. Ankley, and T. Collette. High‐resolution mass spectrometry of skin mucus for monitoring physiological impacts and contaminant biotransformation products in fathead minnows exposed to wastewater effluent. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 37(3): 788-796, (2018).

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Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020

Metadata Source

Harvested from EPA ScienceHub

Additional Metadata

Resource Type Dataset
Metadata Created Date November 12, 2020
Metadata Updated Date November 12, 2020
Publisher U.S. EPA Office of Research and Development (ORD)
Data Last Modified 2017-05-16
Public Access Level public
Bureau Code 020:00
Schema Version
Harvest Object Id 0b5fcae7-ade6-4836-b1ba-9763c9271e05
Harvest Source Id 04b59eaf-ae53-4066-93db-80f2ed0df446
Harvest Source Title EPA ScienceHub
Program Code 020:095
Publisher Hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
Related Documents
Source Datajson Identifier True
Source Hash 5a102930795ac55b666b4fe0d44d673d1ba2333b
Source Schema Version 1.1

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