Hawaiian hoary bats ('ōpe'ape'a; Lasiurus semotus) were surveyed at 23 sites on Hawaiʻi Island from 33 to 2,341 m elevation from May 2018 to August 2021. Of the 23 sites, 8 were established as fixed survey sites for sampling at repeated intervals from January 2019 through January 2021. We surveyed each fixed site at least once per four month period (January–April, May–August, September–December), with a survey comprising one to three netting events. Additional opportunistic surveys were conducted at alternate locations or on alternate dates. We captured 138 unique bats (37 female, 101 male) and recaptured 10 bats over 224 mist-netting events. This data file includes data pertaining to mist netting effort locations, dates, times, use of acoustic playbacks, total survey time, and net sizes.