It is unclear whether DNA (Deoxyribonucleic Acid) lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data release consists of three tabular datasets of raw absorbance measurements that were collected with a BioRad Model 3550 ELISA (Enzyme linked Immunosorbant Assay) plate reader at a wavelength setting of 405 nm from serial dilutions of whole blood that were preserved with either PBS (phosphate buffered saline) or two different DNA lysis buffers and a single tabular dataset of Percent ELISA Values that were derived from the raw absorbance measurements. The whole blood was collected from uninfected Hawaii Amakihi and Hawaii Amakihi experimentally infected with Plasmodium relictum that were collected from Upper Waikea Forest Reserve on Hawaii Island.