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Hawaii Island forest bird avian malaria detection using whole blood preserved in lysis buffer, 2005-2006

Metadata Updated: July 6, 2024

It is unclear whether DNA (Deoxyribonucleic Acid) lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data release consists of three tabular datasets of raw absorbance measurements that were collected with a BioRad Model 3550 ELISA (Enzyme linked Immunosorbant Assay) plate reader at a wavelength setting of 405 nm from serial dilutions of whole blood that were preserved with either PBS (phosphate buffered saline) or two different DNA lysis buffers and a single tabular dataset of Percent ELISA Values that were derived from the raw absorbance measurements. The whole blood was collected from uninfected Hawaii Amakihi and Hawaii Amakihi experimentally infected with Plasmodium relictum that were collected from Upper Waikea Forest Reserve on Hawaii Island.

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024

Metadata Source

Harvested from DOI EDI

Additional Metadata

Resource Type Dataset
Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024
Publisher U.S. Geological Survey
Identifier USGS:5de59150e4b02caea0e8fbb1
Data Last Modified 20200827
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context
Metadata Catalog ID
Schema Version
Catalog Describedby
Harvest Object Id 43dcc85f-1607-4691-9423-234340330d0c
Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
Harvest Source Title DOI EDI
Metadata Type geospatial
Old Spatial -156.264,18.8023,-154.682,20.3395
Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
Source Datajson Identifier True
Source Hash de05218124f8d1f538c92dfb76a889b433e723dff890511837da4dbd636c986a
Source Schema Version 1.1
Spatial {"type": "Polygon", "coordinates": -156.264, 18.8023, -156.264, 20.3395, -154.682, 20.3395, -154.682, 18.8023, -156.264, 18.8023}

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