Handheld FRET-Aptamer Sensor for Water Safety, Phase I

Metadata Updated: May 2, 2019

Operational Technologies Corporation (OpTech) proposes to expand its current NASA Phase 2 SBIR handheld fluorometer and bone marker fluorescence resonance energy transfer (FRET)-DNA aptamer assay system to include detection of bacteria, fungi, and parasites that may contaminate astronauts' water supplies. For Gram positive bacteria, teichoic acids and peptidoglycan will serve as targets. For Gram negative bacteria, common lipopolysaccharide moieties such as 2-keto-3-deoxyoctanate (KDO antigen) will be targeted for aptamer development. Similarly, for fungi, cell wall chitin will be used to select highly specific FRET-aptamers from a randomized DNA library. Parasites such as Cryptosporidium and Giardia will require more specific whole cell or surface protein aptamer selection approaches, but OpTech has recently demonstrated detection of 30 E. coli bacteria per ml using such an approach under NSF Phase 1 SBIR funding. Prototype assays will be lyophilized in plastic cuvettes and capped under vacuum or otherwise sealed to prevent opening in negative pressure environments. Lyophilization with trehalose or other excipients will extend shelf-life to greater than 2 years for these rapid (15 minute) one step (homogeneous) FRET assays that will be quantified with an ultrasensitive commercial handheld fluorometer. Data can be displayed on the handheld reader and downloaded to a laptop computer.

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Public: This dataset is intended for public access and use. License: U.S. Government Work

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Metadata Created Date August 1, 2018
Metadata Updated Date May 2, 2019

Metadata Source

Harvested from NASA Data.json

Additional Metadata

Resource Type Dataset
Metadata Created Date August 1, 2018
Metadata Updated Date May 2, 2019
Publisher Space Technology Mission Directorate
Unique Identifier TECHPORT_9079
Maintainer Email
Public Access Level public
Bureau Code 026:00
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://data.nasa.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Datagov Dedupe Retained 20190501230127
Harvest Object Id 038c5446-db0d-4138-90ff-552cb76463d8
Harvest Source Id 39e4ad2a-47ca-4507-8258-852babd0fd99
Harvest Source Title NASA Data.json
Data First Published 2010-07-01
Homepage URL https://techport.nasa.gov/view/9079
License http://www.usa.gov/publicdomain/label/1.0/
Data Last Modified 2018-07-19
Program Code 026:027
Source Datajson Identifier True
Source Hash 76228bf8ea8f3b4b904343022bba8b476ae77e98
Source Schema Version 1.1

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