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Genetic Data Used to Develop a Genotyping-in-Thousands by Sequencing (GT-seq) Panel for Identifying Individuals and Estimating Relatedness in Alaska Black Bears (Ursus americanus)

Published by U.S. Geological Survey | Department of the Interior | Catalog Last Checked: May 05, 2026 at 07:49 PM | Dataset Last Updated: April 14, 2025 at 12:00 AM
This data package includes genotype data for two independent sets of American black bear (Ursus americanus) samples. The first set of samples was collected from 85 adult bears harvested by permitted hunters in southcentral Alaska from 1994-2019; we hereafter refer to these as "hunter-harvested" samples. These hunter-harvested samples were genotyped with restriction site-associated sequencing (RAD-seq) and were used to detect sequence variants (microhaplotypes and SNPs) with high heterozygosity in Alaska bear populations, and design primers for 'genotyping-in-thousands by sequencing' (GT-seq). The second set of samples includes 79 of the hunter-harvested samples, as well as 84 additional samples collected from adult and juvenile bears on the Joint Base Elmendorf-Richardson (JBER) in Anchorage, Alaska, from 2020 to 2022. These samples were genotyped at 170 microhaplotype loci using GT-seq.

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