Invertebrate, fish, and habitat data were collected as part of a cooperative project that began in 2002 between the U.S. Geological Survey, Colorado Springs Utilities, and Colorado Springs Engineering. Other entities have contributed to the project over the years including Colorado Parks and Wildlife. Fish samples were collected and analyzed at 12 sites in the Fountain Creek Basin, El Paso and Pueblo Counties, Colorado. The number of sites sampled each year as well as the number of annual visits during which data were collected, and sampling methods used during each visit vary. The fish data were collected between 2003 and 2023 and is intended to be updated annually as new data are collected and available. Users of these data are advised to read all metadata and associated documentation thoroughly to understand appropriate use and data limitations. NOTE: Although previous versions are available from the author, all the records in previous versions can be found in version 4.0. First posted - January 7, 2022 (available from author); Revised - May, 2022 (version 2.0); Revised - March, 2023 (version 3.0); Revised - March, 2024 (version 4.0).