These data consists of three related tables describing in-situ field conditions including pesticide concentration, water quality, and post-treatment survival of crayfish associated with the application of a pesticide to a stormwater retention pond and burrows to suppress or eradicate an invasive crayfish species, Procambarus clarkii, in support of high-priority research developing control methods to mitigate impacts of invasive crayfish within the Great Lakes Basin. Effectiveness of the treatment was accessed using an in-situ bioassay and by measuring pesticide concentrations in water, sediment, and caged crayfish. Water quality data, including temperature, pH, specific conductance, dissolved oxygen, alkalinity, hardness, ammonia, and turbidity, in stormwater ponds was collected to evaluate whether environmental conditions may impact treatment effectiveness and persistence of pesticide. Pesticide concentrations in pond water were assessed prior to chemical application of the treatment pond and monitored for 88 days post application. Pesticide concentrations in burrows and adjacent pond were monitored prior to treating burrows and for up to three days post application. Research will assist management and regulatory agencies in interpretating laboratory acute and chronic data relative to field-based treatment and effects data, and in developing permitting requirements and best management practices for open-water and burrowed invasive crayfish populations.