These data consist of three related tables describing test conditions including pesticide concentration, water quality, and post-treatment survival of crayfish associated with laboratory studies conducted to determine 24-hr acute lethal concentrations of two pesticides containing pyrethrin or cypermethrin for two crayfish species, Procambarus clarkii and Faxonius virilis. Tests investigated whether two nominal temperatures (10 and 22 °C) and total suspended solids in water affected pesticide toxicity. Laboratory data will be used to design field treatments for open-water populations of invasive P. clarkii. Water quality monitoring during testing followed standard testing protocols and included temperature, pH, specific conductance, dissolved oxygen, alkalinity, hardness, ammonia, turbidity, and total suspended solids in test jars with sediment from West Bearskin Lake, MN (about 2% Total Organic Carbon (TOC)). Water samples were collected from water treatments with and without sediment for pesticide concentrations. Pesticide concentrations are reported for water and for filters plus sediment. Test organisms were exposed to pesticides for 24-hr prior before being assessed for survival.