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    • Experimental tests of the effects of OA on early life history of marine fishes

    Metadata Updated: October 19, 2024

    These data characterize treatments and outcomes in laboratory-based ocean acidification experiments conducted that the NOAA NEFSC Howard Laboratory. Experiments vary by species used, scope of study (experiment duration and species’ life-stages examined), and experimental design including number of treatments, levels, and replicates, and the suite of response variables. This experiment used summer flounder (Paralichthys dentatus) as subjects, one treatment (concentration of aqueous pCO2), three treatment levels (775, 1808, and 4714 uatm pCO2, pHs of 7.8, 7.5, and 7.1, respectively), three replicates (replicated downstream from each unique CO2 source), and the following response variables: survival of embryos to hatching and of larvae to age 28 d post-hatching; size, shape, and image-based developmental features of larvae; and histopathologically determined status of key organs (eye, heart, liver, gall bladder, gastro-intestinal tract, epidermis, kidney, spinal cord, and muscles) and cranio-facial and skeletal elements.

    Access & Use Information

    License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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    Dates

    Metadata Date February 29, 2024
    Metadata Created Date October 19, 2024
    Metadata Updated Date October 19, 2024
    Reference Date(s) (publication)
    Frequency Of Update notPlanned

    Metadata Source

    Harvested from NMFS NEFSC

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Date February 29, 2024
    Metadata Created Date October 19, 2024
    Metadata Updated Date October 19, 2024
    Reference Date(s) (publication)
    Responsible Party (Point of Contact, Custodian)
    Contact Email
    Guid gov.noaa.nmfs.inport:27478
    Access Constraints Cite As: Northeast Fisheries Science Center, [Date of Access]: Experimental tests of the effects of OA on early life history of marine fishes [Data Date Range], https://www.fisheries.noaa.gov/inport/item/27478., Access Constraints: NEFSC Data Access Constraint: If data are stored on an internal NEFSC network, the requester may not have direct access to query the data unless they are also authorized for network access.
    Coupled Resource
    Frequency Of Update notPlanned
    Harvest Object Id 09aeebb3-6d0e-4296-9140-797907ee9bc2
    Harvest Source Id 523189fa-8edc-48e1-9ec5-f571e7dd923c
    Harvest Source Title NMFS NEFSC
    Licence NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.
    Lineage Data were collected by pre-planned design and varied with experiment. Data on embryo deaths and hatchlings were collected at a daily frequency; size and shape data of hatchlings (Nmax = 10 per replicate) were collected from the interquartile range of the hatch frequency distribution once hatching commenced; mortality of larvae was checked daily; larvae were sampled for image-based size and shape every 7 days through 28 days post-hatching. Data from images were scored via image analysis software (Image Tool v.3). Histopathology data were collected from preserved larvae (staining sequence in Chambers et al. 2014 ibid.). All data were entered into Excel spreadsheets.
    Metadata Language eng
    Metadata Type geospatial
    Progress completed
    Spatial Data Service Type
    Spatial Reference System
    Spatial Harvester True
    Temporal Extent Begin 2010
    Temporal Extent End 2014

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