From 2017-2019, the Upper Midwest Environmental Sciences Center (UMESC) analyzed microcystin concentrations in samples collected from three different studies. The first study was on the movement and distribution of invasive carp (Bighead Carp, Silver Carp, Grass Carp) in the upper Mississippi River between lock and dam 16 and lock and dam 19. Samples were collected from May through October of 2017 and 2018 from backwaters, impounded areas and main channel areas in this reach of the Mississippi River. The second study was a nutrient and metal amendment study performed on natural phytoplankton communities from Lake Erie and Lake Michigan. This was a laboratory study where natural phytoplankton communities were incubated for 72 hours with amendments of ammonium, phosphate and metals (iron, zinc, molybdenum, nickel and manganese). After 72 hours, communities were sampled for microcystin concentration (among other metrics not reported here). The third study was a nutrient diffusing substrate study, where periphyton were grown on suspended substrates that leached nutrients or metals. After two weeks of deployment periphyton was collected from the substrates, diluted in purified water and then analyzed for microcystin concentration. Microcystin concentrations for all experiments were estimated using enzyme-linked immunosorbent assay (ELISA) test kits. We used a Bayesian method to calibrate the absorbance data from the kit and report here on both the microcystin concentrations of the samples analyzed, but also report the raw absorbance data from both samples and calibration standards so that others could recreate the microcystin analysis using other methods if they so choose.