Establishing sex ratios of sea turtle foraging populations: validation of a novel testosterone hormone assay technology and sex assessment for five species.

Metadata Updated: January 17, 2017

Demographic data are essential for developing sound management and conservation plans for marine turtle populations. Sex ratios, even though they are an essential demographic parameter, have been determined at very few foraging grounds. Understanding the sex ratio of foraging populations is essential for estimating spatially explicit threats to, and survivorship of each sex. Sea turtles do not have sex chromosomes and because physical signs of secondary sex characteristics (i.e. males have longer tails) do not occur until a turtle reaches maturity, analysis of testosterone (T) concentration in blood plasma is one method used to determine the sex of juvenile turtles. The project involved (1) validation (in-house and cross-laboratory) of an enzyme-linked immunosorbent assay (ELISA) Testosterone hormone assay for 5 turtle species, (2) creation of a database to collate turtle hormone data, and (3) development of a model to provide probabilities of unknown juvenile green turtle sex. We validated the ELISA for use with green, loggerhead and Kemp's ridley sea turtles and expect validations for hawksbill and leatherback turtles to be complete by the end of April 2015. The turtle hormone database was created in Sequel Server with an Access front and data analysis was automated using queries in Access. The model to predict unknown green turtle sex was created and incorporated into a manuscript that was submitted for publication.

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Metadata Date 2016-02-27T06:39:27.291-05:00
Metadata Created Date March 7, 2016
Metadata Updated Date January 17, 2017
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Harvested from NOAA CSW Harvest Source

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Resource Type Dataset
Metadata Date 2016-02-27T06:39:27.291-05:00
Metadata Created Date March 7, 2016
Metadata Updated Date January 17, 2017
Reference Date(s) (publication)
Responsible Party Southwest Fisheries Science Center (Point of Contact)
Contact Email
Access Constraints Access Constraints: None | Use Constraints: Disclaimer - While every effort has been made to ensure that these data are accurate and reliable within the limits of the current state of the art, NOAA cannot assume liability for any damages caused by any errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty.
Coupled Resource
Frequency Of Update
Guid gov.noaa.nmfs.inport:24174
Harvest Object Id 716d7cd1-958c-42d3-832e-50a517f0f119
Harvest Source Id 2aed8e29-fc5b-4cde-aa66-fb1118fd705e
Harvest Source Title NOAA CSW Harvest Source
Licence
Metadata Language eng
Metadata Type geospatial
Progress onGoing
Spatial Data Service Type
Spatial Reference System
Spatial Harvester True
Temporal Extent Begin 2014

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