This data set summarizes electrofishing effort, observations, and tissue sampling of aquatic vertebrates near Yellow Pine, Idaho. Sampling was conducted using a backpack electrofishing unit at two reference sites including Cane Creek and Sugar Creek, and at three mine impact sites including Sugar Creek and Cinnabar Creek. Juvenile Bull Trout (Salvelinus confluentus) were present at all five sites and were the primary species targeted during our sampling effort. Additional tissue samples of Sculpin, and amphibians were taken when available. Where possible, a two pass (quantitative depletion) electrofishing survey was conducted at each site for non-USGS stakeholders (Nez Perce Tribe and Idaho Department of Fish and Game) to estimate population density if necessary to fit project needs. Electrofishing efforts were immediately halted when adult spawning Bull Trout were encountered so as to minimize harm to that species and in those cases a two pass quantitative depletion survey was not completed. Both summarized and site specific data included are in tabular form. Data is presented in both .csv and .xlsx format, the content within the Excel workbook is the same content within each individual .csv and .jpeg.