Effect of Holstein genotype on immune response to an intramammary Escherichia coli challenge

Metadata Updated: June 5, 2025

Since 1964, the University of Minnesota has selectively bred a Holstein herd to maintain genetically static, unselected Holsteins (UH). Comparison of these UH cows with contemporary Holstein (CH) has demonstrated that the UH cows not only produce less milk but also have fewer health concerns than their CH herdmates. The objective of this study was to determine the effects of Holstein genotype on innate immune response in an experimental intramammary E. coli challenge model. Primiparous UH (n= 5) and CH (n = 7) cows received 430 cfu E. coli strain P4 in 1 quarter. Blood samples were collected at 0, 0.5, 1, 2, 7d relative to E. coli infusion. RNA Sequencing was conducted using the HiSeq 2500 platform (Illumina, San Diego, CA) to generate an average of 46.96 ± 2.67 million 100 bp paired end reads per sample. For more information about this dataset contact: John Lippolis: john.lippolis@usda.gov Hao Ma: hao.ma@usda.gov Resources in this dataset:Resource Title: Effect of Holstein genotype on immune response to an intramammary Escherichia coli challenge. File Name: Supplemental Table 1.csvResource Description: The results of RNA sequencing analysis. The raw sequencing data were submitted to NCBI SRA database with reference PRJNA808034.

Access & Use Information

Public: This dataset is intended for public access and use. License: us-pd

Downloads & Resources

Supplemental Table 1.csvCSV
- Data.gov Preview
Download

Dates

Metadata Created Date	March 30, 2024
Metadata Updated Date	June 5, 2025
Data Update Frequency	R/PT1S

Metadata Source

Data.json Data.json Metadata
Download Metadata

Harvested from USDA JSON

Additional Metadata

Resource Type	Dataset
Metadata Created Date	March 30, 2024
Metadata Updated Date	June 5, 2025
Publisher	Agricultural Research Service
Maintainer	Lippolis, John
Identifier	10.15482/USDA.ADC/1524725
Data Last Modified	2025-05-01
Public Access Level	public
Data Update Frequency	R/PT1S
Bureau Code	005:18
Metadata Context	https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version	https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby	https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id	efd467a8-7123-42bf-a0fa-27c404ecc12f
Harvest Source Id	d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title	USDA JSON
License	https://www.usa.gov/publicdomain/label/1.0/
Old Spatial	{"type": "MultiPolygon", "coordinates": -91.368417, 43.501391, -91.215062, 43.501391, -91.204109, 43.353514, -91.056231, 43.254929, -91.176724, 43.134436, -91.143862, 42.909881, -91.067185, 42.75105, -90.711184, 42.636034, -90.639984, 42.510065, -90.420906, 42.329326, -90.393521, 42.225264, -90.168967, 42.126679, -90.141582, 42.000709, -90.179921, 41.809016, -90.311367, 41.743293, -90.344229, 41.589939, -90.656414, 41.463969, -91.045277, 41.414677, -91.111001, 41.239415, -90.946692, 41.097014, -90.963123, 40.921752, -91.09457, 40.823167, -91.121954, 40.669813, -91.401278, 40.560274, -91.417709, 40.379535, -91.527248, 40.412397, -91.729895, 40.615043, -91.833957, 40.609566, -93.257961, 40.582182, -94.632673, 40.571228, -95.7664, 40.587659, -95.881416, 40.719105, -95.826646, 40.976521, -95.925231, 41.201076, -95.919754, 41.453015, -96.095016, 41.540646, -96.122401, 41.67757, -96.062155, 41.798063, -96.127878, 41.973325, -96.264801, 42.039048, -96.44554, 42.488157, -96.631756, 42.707235, -96.544125, 42.855112, -96.511264, 43.052282, -96.434587, 43.123482, -96.560556, 43.222067, -96.527695, 43.397329, -96.582464, 43.479483, -96.451017, 43.501391, -91.368417, 43.501391, -92.014696, 46.705401, -92.091373, 46.749217, -92.29402, 46.667063, -92.29402, 46.075553, -92.354266, 46.015307, -92.639067, 45.933153, -92.869098, 45.719552, -92.885529, 45.577151, -92.770513, 45.566198, -92.644544, 45.440228, -92.75956, 45.286874, -92.737652, 45.117088, -92.808852, 44.750133, -92.545959, 44.569394, -92.337835, 44.552964, -92.233773, 44.443425, -91.927065, 44.333886, -91.877772, 44.202439, -91.592971, 44.032654, -91.43414, 43.994316, -91.242447, 43.775238, -91.269832, 43.616407, -91.215062, 43.501391, -91.368417, 43.501391, -96.451017, 43.501391, -96.451017, 45.297827, -96.681049, 45.412843, -96.856311, 45.604536, -96.582464, 45.818137, -96.560556, 45.933153, -96.598895, 46.332969, -96.719387, 46.437031, -96.801542, 46.656109, -96.785111, 46.924479, -96.823449, 46.968294, -96.856311, 47.609096, -97.053481, 47.948667, -97.130158, 48.140359, -97.16302, 48.545653, -97.097296, 48.682577, -97.228743, 49.000239, -95.152983, 49.000239, -95.152983, 49.383625, -94.955813, 49.372671, -94.824366, 49.295994, -94.69292, 48.775685, -94.588858, 48.715438, -94.260241, 48.699007, -94.221903, 48.649715, -93.838517, 48.627807, -93.794701, 48.518268, -93.466085, 48.545653, -93.466085, 48.589469, -93.208669, 48.644238, -92.984114, 48.62233, -92.726698, 48.540176, -92.655498, 48.436114, -92.50762, 48.447068, -92.370697, 48.222514, -92.304974, 48.315622, -92.053034, 48.359437, -92.009219, 48.266329, -91.713464, 48.200606, -91.713464, 48.112975, -91.565587, 48.041775, -91.264355, 48.080113, -91.083616, 48.178698, -90.837154, 48.238944, -90.749522, 48.091067, -90.579737, 48.123929, -90.377091, 48.091067, -90.141582, 48.112975, -89.873212, 47.987005, -89.615796, 48.008913, -89.637704, 47.954144, -89.971797, 47.828174, -90.437337, 47.729589, -90.738569, 47.625527, -91.171247, 47.368111, -91.357463, 47.20928, -91.642264, 47.028541, -92.091373, 46.787555, -92.014696, 46.705401}
Program Code	005:040
Source Datajson Identifier	True
Source Hash	4b9c01ee64b24d4dbb635f49c238b3b47e30f5c75dc1635ea9ee1193d64e8faa
Source Schema Version	1.1
Spatial	{"type": "MultiPolygon", "coordinates": -91.368417, 43.501391, -91.215062, 43.501391, -91.204109, 43.353514, -91.056231, 43.254929, -91.176724, 43.134436, -91.143862, 42.909881, -91.067185, 42.75105, -90.711184, 42.636034, -90.639984, 42.510065, -90.420906, 42.329326, -90.393521, 42.225264, -90.168967, 42.126679, -90.141582, 42.000709, -90.179921, 41.809016, -90.311367, 41.743293, -90.344229, 41.589939, -90.656414, 41.463969, -91.045277, 41.414677, -91.111001, 41.239415, -90.946692, 41.097014, -90.963123, 40.921752, -91.09457, 40.823167, -91.121954, 40.669813, -91.401278, 40.560274, -91.417709, 40.379535, -91.527248, 40.412397, -91.729895, 40.615043, -91.833957, 40.609566, -93.257961, 40.582182, -94.632673, 40.571228, -95.7664, 40.587659, -95.881416, 40.719105, -95.826646, 40.976521, -95.925231, 41.201076, -95.919754, 41.453015, -96.095016, 41.540646, -96.122401, 41.67757, -96.062155, 41.798063, -96.127878, 41.973325, -96.264801, 42.039048, -96.44554, 42.488157, -96.631756, 42.707235, -96.544125, 42.855112, -96.511264, 43.052282, -96.434587, 43.123482, -96.560556, 43.222067, -96.527695, 43.397329, -96.582464, 43.479483, -96.451017, 43.501391, -91.368417, 43.501391, -92.014696, 46.705401, -92.091373, 46.749217, -92.29402, 46.667063, -92.29402, 46.075553, -92.354266, 46.015307, -92.639067, 45.933153, -92.869098, 45.719552, -92.885529, 45.577151, -92.770513, 45.566198, -92.644544, 45.440228, -92.75956, 45.286874, -92.737652, 45.117088, -92.808852, 44.750133, -92.545959, 44.569394, -92.337835, 44.552964, -92.233773, 44.443425, -91.927065, 44.333886, -91.877772, 44.202439, -91.592971, 44.032654, -91.43414, 43.994316, -91.242447, 43.775238, -91.269832, 43.616407, -91.215062, 43.501391, -91.368417, 43.501391, -96.451017, 43.501391, -96.451017, 45.297827, -96.681049, 45.412843, -96.856311, 45.604536, -96.582464, 45.818137, -96.560556, 45.933153, -96.598895, 46.332969, -96.719387, 46.437031, -96.801542, 46.656109, -96.785111, 46.924479, -96.823449, 46.968294, -96.856311, 47.609096, -97.053481, 47.948667, -97.130158, 48.140359, -97.16302, 48.545653, -97.097296, 48.682577, -97.228743, 49.000239, -95.152983, 49.000239, -95.152983, 49.383625, -94.955813, 49.372671, -94.824366, 49.295994, -94.69292, 48.775685, -94.588858, 48.715438, -94.260241, 48.699007, -94.221903, 48.649715, -93.838517, 48.627807, -93.794701, 48.518268, -93.466085, 48.545653, -93.466085, 48.589469, -93.208669, 48.644238, -92.984114, 48.62233, -92.726698, 48.540176, -92.655498, 48.436114, -92.50762, 48.447068, -92.370697, 48.222514, -92.304974, 48.315622, -92.053034, 48.359437, -92.009219, 48.266329, -91.713464, 48.200606, -91.713464, 48.112975, -91.565587, 48.041775, -91.264355, 48.080113, -91.083616, 48.178698, -90.837154, 48.238944, -90.749522, 48.091067, -90.579737, 48.123929, -90.377091, 48.091067, -90.141582, 48.112975, -89.873212, 47.987005, -89.615796, 48.008913, -89.637704, 47.954144, -89.971797, 47.828174, -90.437337, 47.729589, -90.738569, 47.625527, -91.171247, 47.368111, -91.357463, 47.20928, -91.642264, 47.028541, -92.091373, 46.787555, -92.014696, 46.705401}
Temporal	2018-09-01/2018-09-30

Didn't find what you're looking for? Suggest a dataset here.

Data Catalog