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DNA Sequencing of Selected Bacterial Growths in Samples from the Madera/Chowchilla-Kings Domestic Aquifer Study unit, 2014

Metadata Updated: July 6, 2024

These data describe microbiological analyses performed on groundwater samples from domestic drinking water supply collected from 42 groundwater wells in the Central Valley of California. Samples were collected between January 2014 and April 2014 for the Groundwater Ambient Monitoring and Assessment (GAMA) program priority basin assessment of the Madera, Chowchilla, and Kings (MACK) groundwater sub-basins’ shallow aquifers. A total of 75 wells were sampled for the MACK study unit between August 2013 and April 2014. Samples for this dataset were vacuum filtered and plated on MI and mEI agars prior to incubation to promote colony growth. Colonies were tallied by their species into columns for various fecal indicator bacteria (FIBs): total coliforms (TCs), Escherichia coli (E. coli), enterococci. Non-target growths were also counted and tallied. Six additional replicate samples were collected for quality assurance. Of the 579 total FIB colonies detected, 106 were selected for polymerase chain reaction (PCR) analysis with the goal of sequencing their DNA. Selected colonies consisted of both target and non-target growths and were taken from 14 samples collected at 13 different wells. DNA sequencing was successful for 34 of the sampled colonies out of a total of 59 submitted. Results for these analyses were reported in FASTA format with the number of bases and their starting position indicated for each batch.

Access & Use Information

Public: This dataset is intended for public access and use. License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024

Metadata Source

Harvested from DOI EDI

Additional Metadata

Resource Type Dataset
Metadata Created Date June 1, 2023
Metadata Updated Date July 6, 2024
Publisher U.S. Geological Survey
Identifier USGS:5e85e5ebe4b01d50927ea4f4
Data Last Modified 20210622
Category geospatial
Public Access Level public
Bureau Code 010:12
Metadata Context
Metadata Catalog ID
Schema Version
Catalog Describedby
Harvest Object Id 39921065-586a-42be-901e-4563c38aeae8
Harvest Source Id 52bfcc16-6e15-478f-809a-b1bc76f1aeda
Harvest Source Title DOI EDI
Metadata Type geospatial
Old Spatial -120.6,36.22,-119.1,37.27
Publisher Hierarchy White House > U.S. Department of the Interior > U.S. Geological Survey
Source Datajson Identifier True
Source Hash ba3a4193cd0842e49b7edb66a245ff242b168cdd94d8d9036dc288069b99a1be
Source Schema Version 1.1
Spatial {"type": "Polygon", "coordinates": -120.6, 36.22, -120.6, 37.27, -119.1, 37.27, -119.1, 36.22, -120.6, 36.22}

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