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Differential proteomic analysis of the massive star coral Orbicella faveolata exposed to experimentally elevated temperatures (NCEI Accession 0242879)

Metadata Updated: November 1, 2023

An inshore-to-offshore gradient in reef coral resilience to high temperatures exists in the Upper Florida Keys, with massive corals of the highly turbid nearshore reefs demonstrating higher capacity to resist summer bleaching than offshore conspecifics. After corroborating this trend with diverse Orbicella faveolata genotypes exposed to elevated temperatures in the laboratory, a proteomic analysis was taken with a subset of 20 samples via iTRAQ labeling followed by nano-liquid chromatography+mass spectrometry; 46 host coral and 40 Symbiodiniaceae dinoflagellate proteins passed all stringent quality control criteria, and the partial proteomes of biopsies of 1) healthy controls, 2) sub-lethally-stressed samples, and 3) actively bleaching corals differed significantly from one another. The proteomic data were then used to train predictive models of coral colony bleaching susceptibility, and both generalized multivariate regression and neural network models were capable of doing so with 100% accuracy. Successful future testing of the predictive power of these models in situ will establish the capacity to monitor coral health on a proactive, pre-death timescale.

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License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

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Dates

Metadata Date 2023-03-15T01:24:38Z
Metadata Created Date March 17, 2023
Metadata Updated Date November 1, 2023
Reference Date(s) December 6, 2021 (publication)
Frequency Of Update asNeeded

Metadata Source

Harvested from NOAA/NESDIS/ncei/accessions

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Additional Metadata

Resource Type Dataset
Metadata Date 2023-03-15T01:24:38Z
Metadata Created Date March 17, 2023
Metadata Updated Date November 1, 2023
Reference Date(s) December 6, 2021 (publication)
Responsible Party (Point of Contact)
Contact Email
Guid gov.noaa.nodc:0242879
Access Constraints Cite as: Mayfield, Anderson B. (2021). Differential proteomic analysis of the massive star coral Orbicella faveolata exposed to experimentally elevated temperatures (NCEI Accession 0242879). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0242879. Accessed [date]., Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Bbox East Long -80.50205
Bbox North Lat 24.95375
Bbox South Lat 24.89742
Bbox West Long -80.61573
Coupled Resource
Frequency Of Update asNeeded
Graphic Preview Description Preview graphic
Graphic Preview File https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0242879
Graphic Preview Type PNG
Harvest Object Id 1a722b86-3b03-4569-a097-a11d1a9c0454
Harvest Source Id c084a438-6f6b-470d-93e0-16aeddb9f513
Harvest Source Title NOAA/NESDIS/ncei/accessions
Licence accessLevel: Public
Lineage
Metadata Language eng
Metadata Type geospatial
Old Spatial {"type": "Polygon", "coordinates": [[[-80.61573, 24.89742], [-80.50205, 24.89742], [-80.50205, 24.95375], [-80.61573, 24.95375], [-80.61573, 24.89742]]]}
Progress completed
Spatial Data Service Type
Spatial Reference System
Spatial Harvester True
Temporal Extent Begin 2017-06-25
Temporal Extent End 2017-12-14

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