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Developing DNA Methods to Monitor Invasive Species and Biodiversity in Estuaries - NERRS/NSC(NERRS Science Collaborative)

Metadata Updated: April 15, 2024

This project tested the use of eDNA methods to identify fish communities, assess biodiversity, and detect invasive crabs in different types of estuaries and connected streams. Samples were collected at Apalachicola, Great Bay, He’eai, Hudson, South Slough and Wells Reserves in 2018 and 2019. The project deployed two methods to analyze DNA in water samples: metabarcoding that identifies a wide range of species, and digital droplet Polymerase Chain Reaction (ddPCR) that identifies a single target organism. The team conducted baseline community assessments for fish at five reserve sites and conducted a survey of anadromous fish in a tributary stream at the sixth reserve. The project found that fish community and biodiversity assessments are well suited to eDNA applications, while invasive crabs are much harder to detect because they do not shed much DNA. The project developed protocols and recommendations for the collection, filtering, and extraction of eDNA samples at estuarine sites, and provided information which will support the design of sampling programs for fish communities.

Access & Use Information

License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

Downloads & Resources

Dates

Metadata Date February 29, 2024
Metadata Created Date November 12, 2020
Metadata Updated Date April 15, 2024
Reference Date(s) (publication)
Frequency Of Update notPlanned

Metadata Source

Harvested from NOS OCM

Additional Metadata

Resource Type Dataset
Metadata Date February 29, 2024
Metadata Created Date November 12, 2020
Metadata Updated Date April 15, 2024
Reference Date(s) (publication)
Responsible Party Office for Coastal Management (Resource Provider); (Point of Contact)
Contact Email
Guid gov.noaa.nmfs.inport:54574
Access Constraints Cite As: Office for Coastal Management, [Date of Access]: Developing DNA Methods to Monitor Invasive Species and Biodiversity in Estuaries - NERRS/NSC(NERRS Science Collaborative) [Data Date Range], https://www.fisheries.noaa.gov/inport/item/54574., Access Constraints: None, Use Constraints: Cite this dataset when used as a source: NOAA retains the right to analyze, synthesize and publish summaries of the NERRS/NSC data. The NERRS/NSC retains the right to be fully credited for having collected and process the data. Following academic courtesy standards, the NERR site where the data were collected should be contacted and fully acknowledged in any subsequent publications in which any part of the data are used. The data enclosed within this package/transmission are only as accurate as the quality assurance and quality control procedures that are described in the associated metadata reporting statement allow. The user bears all responsibility for its subsequent use/misuse in any further analyses or comparisons. The Federal government does not assume liability to the Recipient or third persons, nor will the Federal government reimburse or indemnify the Recipient for its liability due to any losses resulting in any way from the use of this data. Requested citation format: NOAA National Estuarine Research Reserve System (NERRS) Science Collaborative(NSC)., Distribution Liability: The distributor does not assume liability.
Bbox East Long -70.82
Bbox North Lat 43.18
Bbox South Lat 42.975
Bbox West Long -70.97
Coupled Resource
Frequency Of Update notPlanned
Harvest Object Id d8e021bd-05a4-427d-8c9c-c20a0b255db0
Harvest Source Id c0121fd9-df15-4168-ac04-42f6e36a794d
Harvest Source Title NOS OCM
Licence NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.
Lineage
Metadata Language eng
Metadata Type geospatial
Old Spatial {"type": "Polygon", "coordinates": [[[-70.97, 42.975], [-70.82, 42.975], [-70.82, 43.18], [-70.97, 43.18], [-70.97, 42.975]]]}
Progress completed
Spatial Data Service Type
Spatial Reference System
Spatial Harvester True
Temporal Extent Begin 2017-11-01
Temporal Extent End 2019-10-01

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