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    • Dataset for Establishing a Reference Focal Plane Using Machine Learning and Beads for Brightfield Imaging

    Metadata Updated: February 1, 2024

    This dataset consists of sets of images corresponding to the data sets 1-8 described in Table 1 in the manuscript "Establishing a Reference Focal Plane Using Machine Learning and Beads for Brightfield Imaging".Data sets from A2K contain two .zip folders: one with the .tiff images and one with the corresponding .txt file with live and dead cell concentration enumeration. The A2K instrument software collects 4 images per acquisition, and each of those images is passed through the A2K instrument's software algorithm which segments the live (green outline), dead (red outline), and debris (yellow outline) objects. Segmentation parameters are set by the user. This creates a total of 8 stored images per acquisition. When in proper focus and brightness, the V100 beads are segmented in green, appearing as live cells. In cases where the beads do not display the bright spot center (when out of focus or too dim) the software may segment the beads in red, as dead cells.Data sets from the Nikon contain .zip folders of .nd2 image stacks that can be opened with Image J.These image sets were used to develop the AI model to identify reference focal plane as described in the associated manuscript.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: See this page for license information.

    Downloads & Resources

    Dates

    Metadata Created Date June 7, 2023
    Metadata Updated Date February 1, 2024

    Metadata Source

    Harvested from NIST

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date June 7, 2023
    Metadata Updated Date February 1, 2024
    Publisher National Institute of Standards and Technology
    Maintainer
    Identifier ark:/88434/mds2-2993
    Data First Published 2023-05-19
    Language en
    Data Last Modified 2024-01-19 00:00:00
    Category Bioscience
    Public Access Level public
    Bureau Code 006:55
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id b5628b54-3e3a-4d86-ab59-fff9927961e1
    Harvest Source Id 74e175d9-66b3-4323-ac98-e2a90eeb93c0
    Harvest Source Title NIST
    Homepage URL https://data.nist.gov/od/id/mds2-2993
    License https://www.nist.gov/open/license
    Program Code 006:045
    Source Datajson Identifier True
    Source Hash bd7c28b75654a5f398dad92c60c42e7a4b9bbc91467f393d44cac48464f85a79
    Source Schema Version 1.1

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