Data from: Toll-like receptor ligands enhance vaccine efficacy against virulent Newcastle disease virus challenge in chickens

Metadata Updated: March 30, 2024

To enhance the efficacy of the current Newcastle disease vaccine, we have tested two potential adjuvants (Imiquimod and ODN-1826) in chickens. Birds were treated with PBS, Imiquimod or ODN-1826 (50 µg/bird) or vaccinated intranasally with live LaSota strain with or without Imiquimod or ODN-1826 (50 µg/bird). Two weeks after vaccination, birds were challenged with virulent New-castle disease virus (chicken/CA/212676/2002). The experimental setting was a laboratory with animal care as approved by the institutional animal care and use committee as appropriate for the species and age of bird. Data are the serum antibody titers to the vaccine or challenge virus as determined by hemagglutination inhibition assay. Virus shedding titers by the oral and cloacal route for individual birds exposed to virulent Newcastle disease virus were determined by quantitative real-time RT-PCR. Expression of antiviral genes from tissues collected at 1 and 3 days after treating 2 week old SPF chickens with adjuvant and/or vaccines were determined by quantitative PCR. Resources in this dataset:

Hemagglutination inhibition (HI) antibody titers 14 days after vaccinating 2-week-old SPF chickens with LaSota vaccine with or without adjuvant (ODN-1826 or Imiquimod) File Name: Lee et al., Serology Data I from Adjuvanted live NDV vaccine study.csv

Hemagglutination inhibition antibody titers 14 days after infecting 4 week old SPF chickens with virulent Newcastle disease virus (California/212676/2002) File Name: Lee et al., Serology Data II from Adjuvanted live NDV vaccine study.csv

Cycle threshold (Ct) value of viral RNA from swabs collected at 2 and 4 days after infecting 4-week-old SPF chickens with virulent Newcastle disease virus (California/212676/2002) File Name: Lee et al.,Virus Shedding Data from Adjuvanted live NDV vaccine study.csv

Cycle threshold (Ct) value of target specific mRNA collected at 1 and 3 days after treating 2-week-old SPF chickens with adjuvant and/or vaccines File Name: Lee et al.,Transcription Data from Adjuvanted live NDV vaccine study.csv

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Public: This dataset is intended for public access and use. License: Creative Commons CCZero

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Dates

Metadata Created Date	March 30, 2024
Metadata Updated Date	March 30, 2024
Data Update Frequency	irregular

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Harvested from USDA JSON

Additional Metadata

Resource Type	Dataset
Metadata Created Date	March 30, 2024
Metadata Updated Date	March 30, 2024
Publisher	Agricultural Research Service
Maintainer	Lee, Chang
Identifier	10.15482/USDA.ADC/1529602
Data Last Modified	2024-02-15
Public Access Level	public
Data Update Frequency	irregular
Bureau Code	005:18
Metadata Context	https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version	https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby	https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id	cc04673c-dbee-456f-8e89-1df988c631b2
Harvest Source Id	d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title	USDA JSON
License	https://creativecommons.org/publicdomain/zero/1.0/
Old Spatial	{"type": "Point", "coordinates": -83.358053863049, 33.928106192905}
Program Code	005:040
Source Datajson Identifier	True
Source Hash	59934a78e8290435a0c4272fc7120d288b934f8e10d39f437e96a5c3cc1931bd
Source Schema Version	1.1
Spatial	{"type": "Point", "coordinates": -83.358053863049, 33.928106192905}

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