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    • Data from: An in vitro analysis of how lactose modifies the gut microbiota structure and function of adults in a donor-independent manner

    Metadata Updated: December 2, 2025

    The impact of lactose on the gut microbiota of healthy adults was examined, using a short-term, in vitro strategy where fecal samples harvested from 18 donors were cultured anaerobically with and without lactose. Donors represented 3 adult age groups. Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883645: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645), RT-qPCR of Bifidobacterium 16S rRNA genes, and short-chain fatty acid concentrations. Resources in this dataset:

    Resource Title: lactose sample attributes File Name: lactose_sample_metadata.csv

    Resource Title: lactose SCFA, pH, and gas production data File Name: lactose_SCFA_data.csv

    Resource Title: Total bacterial and Bifidobacterium genus qPCR File Name: lactose_qPCR_all_bacteria_and_bifido.csv

    Access & Use Information

    Public: This dataset is intended for public access and use. License: Creative Commons Attribution

    Downloads & Resources

    Dates

    Metadata Created Date March 30, 2024
    Metadata Updated Date December 2, 2025

    Metadata Source

    Harvested from USDA JSON

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date March 30, 2024
    Metadata Updated Date December 2, 2025
    Publisher Agricultural Research Service
    Maintainer
    Identifier 10.15482/USDA.ADC/1529150
    Data Last Modified 2025-11-21
    Public Access Level public
    Bureau Code 005:18
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 2f753ce9-80dc-4ce6-be8b-f1fba82074a7
    Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
    Harvest Source Title USDA JSON
    License https://creativecommons.org/licenses/by/4.0/
    Old Spatial {"type": "Point", "coordinates": -75.187092456102, 40.078126249741}
    Program Code 005:040
    Source Datajson Identifier True
    Source Hash 811c3d61acdf869a2e050f79da2c51f30a0af7898160b880b08da1b3eeaa4ca6
    Source Schema Version 1.1
    Spatial {"type": "Point", "coordinates": -75.187092456102, 40.078126249741}
    Temporal 2021-06-01/2021-06-01

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