This dataset provides the results of a national survey of the United States for the salamander chytrid fungus Batrachochytrium salamandrivorans (Bsal) and the amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) conducted by the U.S. Geological Survey Amphibian Research and Monitoring Initiative from May 2014 to August 2017. Sites were sampled by capturing amphibians by hand or by traps or nets that were then swabbed individually using methods that prevent sample contamination. All swabs were then analyzed using a real-time TaqMan PCR for detection of B. salamandrivorans and Bd on the extracted DNA. The data consist of locality information and data on the individual sampled, as well as the result of the test for B. salamandrivorans and Bd. No cases of B. salamandrivorans were detected in this study.