Data and Code: Temperature influences desiccation resistance of bumble bees
In this experiment, we crossed temperature (20, 25, and 30C) with humidity (<5%, 50%, and >95% RH) and measured the effects on bumble bee (Bombus impatiens) water balance. We had 10 replicates per treatment, with 6 source colonies, resulting in a total of 540 bees. We placed bees singly into tubes and checked for mortality every 2 hours for the first 12 hours and then every 4 hours until 5/10 bees from each temperature, humidity, colony treatment had reached mortality (52h and 48h for the two trials). We measured mass before and after mortality to evaluate water loss and then dried bees to a constant dry weight to get the initial water content and dry mass of each bee.
Resources
2 resources available
-
desiccation resistance MASTER.xlsx
XLSX -
Desiccation Resistance.R
R
Find Related Datasets
Search by Tags
Click any tag below to search for similar datasets
Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[
"005:18"
]
|
| contactPoint |
{
"fn": "Botsch, Jamieson C.",
"hasEmail": "mailto:jcbotsch@gmail.com"
}
|
| description | <p dir="ltr">In this experiment, we crossed temperature (20, 25, and 30C) with humidity (<5%, 50%, and >95% RH) and measured the effects on bumble bee (<i>Bombus impatiens</i>) water balance. We had 10 replicates per treatment, with 6 source colonies, resulting in a total of 540 bees. We placed bees singly into tubes and checked for mortality every 2 hours for the first 12 hours and then every 4 hours until 5/10 bees from each temperature, humidity, colony treatment had reached mortality (52h and 48h for the two trials). We measured mass before and after mortality to evaluate water loss and then dried bees to a constant dry weight to get the initial water content and dry mass of each bee. </p> |
| distribution |
[
{
"@type": "dcat:Distribution",
"title": "desiccation resistance MASTER.xlsx",
"format": "xlsx",
"mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
"downloadURL": "https://ndownloader.figshare.com/files/44867044"
},
{
"@type": "dcat:Distribution",
"title": "Desiccation Resistance.R",
"format": "R",
"mediaType": "text/plain",
"downloadURL": "https://ndownloader.figshare.com/files/44867047"
}
]
|
| identifier | 10.15482/USDA.ADC/25345426.v1 |
| keyword |
[
"Bombus impatiens",
"critical water content",
"desiccation resistance",
"water loss rates"
]
|
| license | https://creativecommons.org/licenses/by/4.0/ |
| modified | 2025-05-02 |
| programCode |
[
"005:040"
]
|
| publisher |
{
"name": "Agricultural Research Service",
"@type": "org:Organization"
}
|
| temporal | 2023-09-28/2023-12-10 |
| title | Data and Code: Temperature influences desiccation resistance of bumble bees |
