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    • Conserved B cell signaling, activation, and differentiation in porcine jejunal and ileal Peyer's patches despite distinct immune landscapes

    Metadata Updated: December 2, 2025

    This dataset contains files used in the manuscript, "Conserved B cell signaling, activation, and differentiation in porcine jejunal and ileal Peyer's patches despite distinct immune landscapes" by Wiarda et al. (2023). Samples of jejunum and ileum containing Peyer's patches were collected from each of three, 8-week-old, female pigs and processed for single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (STomics). Data were analyzed to determine cell type compositions, spatial regions, gene expression, signaling interaction networks, activation trajectories, and cell locations. Files included here can be used to reconstruct all analyses of the manuscript, allowing individual users to perform independent queries. Also included is a vignette of all coding steps used in this work, provided as a Markdown file. Scripts and raw data will be available via preprint (to follow). See README for list of resources in this dataset with descriptions.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: Creative Commons Attribution Share-Alike

    Downloads & Resources

    Dates

    Metadata Created Date March 30, 2024
    Metadata Updated Date December 2, 2025

    Metadata Source

    Harvested from USDA JSON

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date March 30, 2024
    Metadata Updated Date December 2, 2025
    Publisher Agricultural Research Service
    Maintainer
    Identifier 10.15482/USDA.ADC/1529557
    Data Last Modified 2025-11-21
    Public Access Level public
    Bureau Code 005:18
    Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Harvest Object Id 66bdafea-e55a-497a-a69a-2bc6bde46739
    Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
    Harvest Source Title USDA JSON
    License https://creativecommons.org/licenses/by-sa/4.0/
    Old Spatial {"type": "Point", "coordinates": -93.576693534851, 42.045130793063}
    Program Code 005:040
    Source Datajson Identifier True
    Source Hash 2a6cfbc671c0dea1b56c059d789cdd678f5adb2e8be41c390a7b2ae077f69177
    Source Schema Version 1.1
    Spatial {"type": "Point", "coordinates": -93.576693534851, 42.045130793063}
    Temporal 2020-04-01/2020-04-01

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