Wild insect pollination has significant positive effects on pollinator-dependent crop production. To assess the spatial distribution of potential wild insect pollination, we mapped the supply of potential wild pollinator habitat (forest, grassland, wetland, and shrubland land cover types) and the demand for pollination (directly pollinator-dependent crops). A foraging travel distance for temperate native bees (1308 meters) was used to estimate relative pollinator activity on cropland based on distance from habitat. We also calculated the proportion of pollinator habitat within pollinator travel distance of crops. This information was summarized by subwatershed (HUC12) to identify regional priority areas for conservation and restoration of pollinator habitat. This shapefile identifies regional conservation priority and restoration priority subwatersheds and includes information on the extent of pollinator-dependent crops, the mean relative pollinator activity on pollinator-dependent crops, and the proportion of pollinator habitat within pollinator flight distance of pollinator-dependent crops by subwatershed for the southeastern United States.