Comparison of the spaceflight transcriptome of four commonly used Arabidopsis thaliana ecotypes

Metadata Updated: March 22, 2019

This experiment compared the spaceflight transcriptomes of four commonly used natural variants (ecotypes) of Arabidopsis thaliana using RNAseq. In nature Arabidopsis is a native of Europe/Asia/Northwestern Africa and is found across the globe growing in a wide range of environments. The geographical spread of these various populations has led to a slow divergence leading to distinct ecotypes. Understanding the impact of this ecotypic variability is an important factor when using Arabidopsis as a model. Seeds of the ecotypes Col_0 Ler-2 Ws-2 and Cvi-0 were flown to the International Space Station as part of CRS-4 mission in the Biological Research in Canister (BRIC) hardware. The seeds were germinated on orbit grown for 8 days and then fixed in RNAlater and frozen in the MELFI freezer for return to Earth. Once returned RNA was isolated and RNAseq performed to catalog the transcriptional patterns of the plants grown in space. An identical set of samples were grown in parallel on the ground to provide controls to allow assessment of transcriptional changes specifically associated with the spaceflight environment. This data release includes 48 out of 56 sample expression files with the remaining 8 files to be released at a later date.

Access & Use Information

Public: This dataset is intended for public access and use. License: U.S. Government Work

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Dates

Metadata Created Date August 1, 2018
Metadata Updated Date March 22, 2019
Data Update Frequency irregular

Metadata Source

Harvested from NASA Data.json

Additional Metadata

Resource Type Dataset
Metadata Created Date August 1, 2018
Metadata Updated Date March 22, 2019
Publisher National Aeronautics and Space Administration
Unique Identifier nasa_genelab_GLDS-37
Maintainer
GeneLab Outreach
Maintainer Email
Public Access Level public
Data Update Frequency irregular
Bureau Code 026:00
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Metadata Catalog ID https://data.nasa.gov/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Datagov Dedupe Retained 20190322035503
Harvest Object Id 72976b18-d080-457c-a6ba-1d67bcb71926
Harvest Source Id 39e4ad2a-47ca-4507-8258-852babd0fd99
Harvest Source Title NASA Data.json
Data First Published 2018-06-26
Homepage URL https://data.nasa.gov/d/n47v-4ngw
License http://www.usa.gov/publicdomain/label/1.0/
Data Last Modified 2018-07-19
Program Code 026:005
Source Datajson Identifier True
Source Hash 7733f509bd6f438344f6003004440b20dd0e91f3
Source Schema Version 1.1
Category Earth Science

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