Fathead minnow whole-mount in situ hybridization (WISH)

Metadata Updated: February 13, 2019

This study demonstrates the potential of whole-mount in situ hybridization (WISH), in conjunction with quantitative real-time polymerase chain reaction (QPCR) assays, to examine the mechanistic basis of the effects of toxicants on early-lifestage fathead minnows. Specifically, fathead minnow embryos were exposed to the environmentally-relevant estrogen receptor agonist, estrone, and the data show that: (1) the estrogen-responsive gene transcripts esr1, vtg, and cyp19b can be up-regulated in very early-lifestages of the fathead minnow, (2) WISH methods developed for zebrafish can also be applied successfully to fathead minnows, and (3) WISH has potential to be a useful tool for toxicological studies pertaining to early-lifestage development in the fathead minnow. This type of mechanistic information relative to spatial distribution of gene expression is important in determining potential biological pathways that may be impacted by targeted chemicals and the development of associated adverse outcome pathways.

This dataset is associated with the following publication: Cavallin, J., A. Schroeder, K. Jensen , D. Villeneuve , B. Blackwell, K. Carlson, M. Kahl , C. LaLone , E. Randolph , and G. Ankley. Evaluation of whole-mount in situ hybridization as a tool for pathway-based toxicological research with early-life stage fathead minnows. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 169: 19-26, (2015).

Access & Use Information

Public: This dataset is intended for public access and use. License: See this page for license information.

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References

https://dx.doi.org/10.1016/j.aquatox.2015.10.002

Dates

Metadata Created Date September 19, 2016
Metadata Updated Date February 13, 2019

Metadata Source

Harvested from EPA ScienceHub

Additional Metadata

Resource Type Dataset
Metadata Created Date September 19, 2016
Metadata Updated Date February 13, 2019
Publisher U.S. EPA Office of Research and Development (ORD)
Unique Identifier A-9w14-314
Maintainer
Gerald Ankley
Maintainer Email
Public Access Level public
Bureau Code 020:00
Schema Version https://project-open-data.cio.gov/v1.1/schema
Harvest Object Id e6736f7b-829d-483b-8555-c9cdf4964f1d
Harvest Source Id cf9b0004-f9fd-420e-bade-a86839e82acf
Harvest Source Title EPA ScienceHub
License https://pasteur.epa.gov/license/sciencehub-license.html
Data Last Modified 2016-09-14
Program Code 020:095
Publisher Hierarchy U.S. Government > U.S. Environmental Protection Agency > U.S. EPA Office of Research and Development (ORD)
Related Documents https://dx.doi.org/10.1016/j.aquatox.2015.10.002
Source Datajson Identifier True
Source Hash 87f426276234d13943dcec1c1c035e057080f17d
Source Schema Version 1.1

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