Skip to content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Skip to content

AFSC/RACE/EcoFOCI: NPRB project number 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flow cytometry cell cycle analysis

Metadata Updated: September 2, 2022

Walleye pollock are an important component of the eastern Bering Sea ecosystem due to their vast numbers and biomass and are of great commercial importance. Their recruitment in the eastern Bering Sea is not well understood but it is largely determined during the first year, so the larval stage may play a significant role. Starvation may contribute to the high and variable mortality that occurs during the larval stage so it is important to monitor and quantify it. This metadata describes data from laboratory and field studies conducted from 2009 to 2012 for NPRB project 926. The project completed the development of an assay using flow cytometric cell cycle analysis to assess the physiological condition of walleye pollock larvae, and applied it to field collected specimens. The use of RNA to improve the accuracy of the assay was also investigated, as well as the relationship between flow cytometric measurements and growth. Results showed that the overall classification accuracy of the assay (healthy or unhealthy) ranged between 75 and 83% depending on the type of cross-validation testing. A nuclear RNA variable (the ratio of the number of S phase nuclei to the number of high nuclear RNA content G1 phase nuclei) improved overall classification accuracy by 11% compared to a model without it. Unhealthy walleye pollock larvae were present in the southeastern Bering Sea in 2009, and this was most likely the result of cold environmental conditions affecting prey availability and larval feeding. In 2010, no unhealthy larvae were detected. This may be the result of less spatial coverage and smaller collections of larvae than in 2009, given that the environmental conditions were similar between years.

Access & Use Information

License: No license information was provided. If this work was prepared by an officer or employee of the United States government as part of that person's official duties it is considered a U.S. Government Work.

Downloads & Resources

Dates

Metadata Date August 9, 2022
Metadata Created Date November 12, 2020
Metadata Updated Date September 2, 2022
Reference Date(s) (publication)
Frequency Of Update notPlanned

Additional Metadata

Resource Type Dataset
Metadata Date August 9, 2022
Metadata Created Date November 12, 2020
Metadata Updated Date September 2, 2022
Reference Date(s) (publication)
Responsible Party (Point of Contact, Custodian)
Contact Email
Guid gov.noaa.nmfs.inport:12673
Access Constraints Cite As: Alaska Fisheries Science Center, [Date of Access]: AFSC/RACE/EcoFOCI: NPRB project number 926: Assessing the condition of walleye pollock, Theragra chalcogramma, larvae in the eastern Bering Sea with muscle-based flow cytometry cell cycle analysis [Data Date Range], https://www.fisheries.noaa.gov/inport/item/12673., Access Constraints: Contact Point Of Contact for data request form. | Use Constraints: User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgement of NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested.
Bbox East Long -163.3
Bbox North Lat 57.4
Bbox South Lat 54.3
Bbox West Long -171.1
Coupled Resource
Frequency Of Update notPlanned
Harvest Object Id 59c1d6e6-d30b-4320-bd25-4ce70400f7cb
Harvest Source Id 26a29bb9-50b0-47fd-920b-edc74aa6ec76
Harvest Source Title NMFS AFSC
Licence NOAA provides no warranty, nor accepts any liability occurring from any incomplete, incorrect, or misleading data, or from any incorrect, incomplete, or misleading use of the data. It is the responsibility of the user to determine whether or not the data is suitable for the intended purpose.
Metadata Language eng
Metadata Type geospatial
Old Spatial {"type": "Polygon", "coordinates": [[[-171.1, 54.3], [-163.3, 54.3], [-163.3, 57.4], [-171.1, 57.4], [-171.1, 54.3]]]}
Progress completed
Spatial Data Service Type
Spatial Reference System
Spatial Harvester True
Temporal Extent Begin 2009
Temporal Extent End 2012

Didn't find what you're looking for? Suggest a dataset here.

data.gov

An official website of the General Services Administration.

Looking for U.S. government information and services?
Visit USA.gov