AFSC/RACE/EcoFOCI: NPRB_1220:Mitochondrial DNA-based identification of eggs, larvae and dietary components of commercially and ecologically important fish species and selected invertebrates in the northeast Pacif

Metadata Updated: February 3, 2017

Accurate identification of various life history stages and prey items of marine fishes and invertebrates is central for understanding distribution,abundance, trophic ecology, and biodiversity of these species. Taxonomic approaches have been successfully applied to ichthyoplankton identification and diet analysis efforts for many years. Identification to the species level requires varying degrees of taxonomic expertise. and diagnostic characters for eggs or larvae in some species have not been elucidated.
In the current dataset we assembled a mitochondrial DNA (mtDNA) database for which used standard laboratory protocols (restriction fragment length polymorphism and Sanger DNA sequencing) to accurately identify any life history stages of selected fish and shrimp species, with special emphasis on those species that have been difficult or impossible to identify by conventional taxonomic means. Fish and shrimp specimens were collected between 2010 - 2013.
We developed a restriction fragment length polymorphism (RFLP)protocol, based upon mitochondrial DNA sequences to distinguish between Pacific halibut and Greenland halibut, but were unable to develop one to discriminate between Bering flounder and flathead sole. We used direct Sanger sequencing of mitochondrial DNA for species identification of 32 species of sculpin and four species of caridean shrimp. PCR products from fish and shrimp samples were sequenced using an ABI 3730 automated sequencer (Applied Biosystems, Inc). DNA sequences from museum voucher specimens were compared with entries in the public databases for those species. Sequences from voucher specimens have greater taxonomic authority for use in species identification than those without vouchers, adding greater confidence to species identifications based upon our data.

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Metadata Date May 18, 2016
Metadata Created Date May 31, 2016
Metadata Updated Date February 3, 2017
Reference Date(s) (publication)
Frequency Of Update notPlanned

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Harvested from NOAA CSW Harvest Source

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Resource Type Dataset
Metadata Date May 18, 2016
Metadata Created Date May 31, 2016
Metadata Updated Date February 3, 2017
Reference Date(s) (publication)
Responsible Party (Point of Contact)
Contact Email
Access Constraints Access Constraints: There are no legal restrictions on access to the data. They reside in public domain and can be freely distributed. | Use Constraints: User must read and fully comprehend the metadata prior to use. Applications or inferences derived from the data should be carefully considered for accuracy. Acknowledgementof NOAA/NMFS/AFSC, as the source from which these data were obtained in any publications and/or other representations of these, data is suggested.
Bbox East Long -120.1
Bbox North Lat 71.5
Bbox South Lat 42.42
Bbox West Long -178.46
Coupled Resource
Frequency Of Update notPlanned
Guid gov.noaa.nmfs.inport:21948
Harvest Object Id 7880167c-88ec-4dec-b2b7-4ee4c8157c7d
Harvest Source Id 2aed8e29-fc5b-4cde-aa66-fb1118fd705e
Harvest Source Title NOAA CSW Harvest Source
Metadata Language eng
Metadata Type geospatial
Progress completed
Spatial Data Service Type
Spatial Reference System
Spatial Harvester True
Temporal Extent Begin 2000
Temporal Extent End 2013

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