Water velocities and discharge were measured in the lock chamber and immediately downstream of Brandon Road Lock on the Des Plaines River at river mile 286 on December 9-10, 2014 using Teledyne RDI Rio Grande 600 and 1200 kHz acoustic Doppler current profilers (ADCP). The data were georeferenced with a differential GPS receiver with submeter accuracy. These ADCP measurements were collected in support of the US Army Corps of Engineers Great Lakes and Mississippi River Interbasin Study (GLMRIS). Velocity measurements were processed using the Velocity Mapping Toolbox (Parsons and others, 2013) to derive temporally- and spatially-averaged water velocity values. Discharge measurements were processed with the QRev discharge computation and review software (Mueller, 2016). Mueller, D.S., 2016, QRev—Software for computation and quality assurance of acoustic Doppler current profiler moving-boat streamflow measurements—User’s manual for version 2.8: U.S. Geological Survey Open-File Report 2016–1052, 50 p., http://dx.doi.org/10.3133/ofr20161052. Parsons, D. R., Jackson, P. R., Czuba, J. A., Engel, F. L., Rhoads, B. L., Oberg, K. A., Best, J. L., Mueller, D. S., Johnson, K. K. and Riley, J. D. (2013), Velocity Mapping Toolbox (VMT): a processing and visualization suite for moving-vessel ADCP measurements. Earth Surf. Process. Landforms, 38: 1244-1260. doi: 10.1002/esp.3367