Field measurements of flow depth and depth-averaged velocity were acquired from a reach of the Tanana River near Nenana, Alaska, July 24, 2019, to support research on estimating surface flow velocities and water depths from remotely sensed data via particle image velocimetry (PIV) and flow resistance equations, respectively. The depth and velocity measurements included in this data release were obtained using a TRDI RiverRay acoustic Doppler current profiler (ADCP) deployed from a boat with an outboard motor. This data release provides depths and depth-averaged flow velocities derived from the raw ADCP data using the TRDI WinRiver II processing software. The WinRiver II output was then imported into the USGS Velocity Mapping Toolbox (VMT), which was used to generate a text file with projected spatial coordinates, depth-averaged velocity magnitudes, and depths. The spatial location of each measurement was obtained using a differential GPS included as part of the RiverRay instrument package. The map projection and datum for these data are UTM Zone 6 N and NAD 83, respectively. This data release consists of a single comma-delimited (*.csv) text file with four columns: UTM_East_m, UTM_North_m, Velocity_Magnitude_m_per_s, Depth_m. The first two columns are the easting and northing spatial coordinates, respectively, the third column is the depth-averaged velocity magnitude in meters per second, and the fourth column is water depth in meters. This field-based data set was used to assess the accuracy of remotely sensed estimates of surface flow velocity and water depth derived from helicopter-based videos via PIV and flow resistance equations, respectively.