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A Sensitivity Analysis of Methodological Variables Associated with Microbiome Measurements

Metadata Updated: December 15, 2023

This repository provides the raw data, analysis code, and results generated during a systematic evaluation of the impact of selected experimental protocol choices on the metagenomic sequencing analysis of microbiome samples. Briefly, a full factorial experimental design was implemented varying biological sample (n=5), operator (n=2), lot (n=2), extraction kit (n=2), 16S variable region (n=2), and reference database (n=3), and the main effects were calculated and compared between parameters (bias effects) and samples (real biological differences). A full description of the effort is provided in the associated publication.

Access & Use Information

Public: This dataset is intended for public access and use. License: See this page for license information.

Downloads & Resources

Dates

Metadata Created Date December 15, 2023
Metadata Updated Date December 15, 2023

Metadata Source

Harvested from NIST

Additional Metadata

Resource Type Dataset
Metadata Created Date December 15, 2023
Metadata Updated Date December 15, 2023
Publisher National Institute of Standards and Technology
Maintainer
Identifier ark:/88434/mds2-3092
Data First Published 2023-11-16
Language en
Data Last Modified 2023-10-05 00:00:00
Category Bioscience:Microbial measurements
Public Access Level public
Bureau Code 006:55
Metadata Context https://project-open-data.cio.gov/v1.1/schema/data.json
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id f86e1d6b-4bb6-4641-8845-be536e8082b0
Harvest Source Id 74e175d9-66b3-4323-ac98-e2a90eeb93c0
Harvest Source Title NIST
Homepage URL https://data.nist.gov/od/id/mds2-3092
License https://www.nist.gov/open/license
Program Code 006:045
Source Datajson Identifier True
Source Hash 2601651561472ac56325c307b871866e3538e1e4ad35235fd8105944ef215fdb
Source Schema Version 1.1

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