S-Layer Nanosheet Binding of Zn and Gd
This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods.
A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000),
References:
Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753.
Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.
Resources
2 resources available
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Gd-Binding S-Layers.xlsx
XLSX -
Zn-Binding S-Layers.xlsx
XLSX
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Complete Metadata
| @type | dcat:Dataset |
|---|---|
| accessLevel | public |
| bureauCode |
[ "019:20" ] |
| contactPoint |
{ "fn": "Caroline Ajo-Franklin", "@type": "vcard:Contact", "hasEmail": "mailto:cajo-franklin@lbl.gov" } |
| dataQuality |
true
|
| description | This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods. A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000), References: Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753. Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886. |
| distribution |
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| DOI | 10.15121/1372392 |
| identifier | https://data.openei.org/submissions/7119 |
| issued | 2016-04-15T06:00:00Z |
| keyword |
[ "Gadolinium", "S-layer", "binding", "brine study", "chemistry", "energy", "gd3", "geothermal", "ion", "metal-binding domain", "mineral recovery", "nanosheet", "synthetic biology", "zinc", "zn2" ] |
| landingPage | https://gdr.openei.org/submissions/951 |
| license | https://creativecommons.org/licenses/by/4.0/ |
| modified | 2020-01-09T00:11:19Z |
| programCode |
[ "019:006" ] |
| projectLead | Holly Thomas |
| projectNumber | FY15 AOP 2514 |
| projectTitle | Engineering Thermophilic Microorganisms to Selectively Extract Strategic Metals from Low Temperature Geothermal Brines |
| publisher |
{ "name": "Lawrence Berkeley National Laboratory", "@type": "org:Organization" } |
| spatial |
"{"type":"Polygon","coordinates":[[[-180,-83],[180,-83],[180,83],[-180,83],[-180,-83]]]}"
|
| title | S-Layer Nanosheet Binding of Zn and Gd |
