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    • Rare Earth Desorption Test with Citrate and Bicarbonate

    Metadata Updated: June 25, 2021

    LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.

    Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.

    Access & Use Information

    Public: This dataset is intended for public access and use. License: Creative Commons Attribution

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    Dates

    Metadata Created Date June 24, 2021
    Metadata Updated Date June 25, 2021

    Metadata Source

    Harvested from OpenEI data.json

    Other Data Resources

    Additional Metadata

    Resource Type Dataset
    Metadata Created Date June 24, 2021
    Metadata Updated Date June 25, 2021
    Publisher Lawrence Livermore National Laboratory
    Maintainer
    Doi 10.15121/1452718
    Identifier https://data.openei.org/submissions/3641
    Data First Published 2017-06-01T06:00:00Z
    Data Last Modified 2018-06-14T16:28:49Z
    Public Access Level public
    Bureau Code 019:20
    Metadata Context https://openei.org/data.json
    Metadata Catalog ID https://openei.org/data.json
    Schema Version https://project-open-data.cio.gov/v1.1/schema
    Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
    Data Quality True
    Harvest Object Id 5ccc7db3-5a8f-430b-a92f-e0adb00d5721
    Harvest Source Id 7cbf9085-0290-4e9f-bec1-91653baeddfd
    Harvest Source Title OpenEI data.json
    Homepage URL https://gdr.openei.org/submissions/965
    License https://creativecommons.org/licenses/by/4.0/
    Old Spatial {"type":"Polygon","coordinates":-122.2433640625,35.15607354867,-118.3872640625,35.15607354867,-118.3872640625,39.295415290912,-122.2433640625,39.295415290912,-122.2433640625,35.15607354867}
    Program Code 019:006
    Projectlead Holly Thomas
    Projectnumber LLNL FY17 AOP 2.5.1.12
    Projecttitle Extraction of Rare Earth Metals from Geothermal Fluids using Bioengineered Microbes
    Source Datajson Identifier True
    Source Hash ba2564ad9d1adb9e177e941575742941dfb3b8ba
    Source Schema Version 1.1
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