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formalin effects data

Published by U.S. EPA Office of Research and Development (ORD) | U.S. Environmental Protection Agency | Catalog Last Checked: April 21, 2026 at 08:00 PM | Dataset Last Updated: April 21, 2020
RNA sequencing data from paired: (1) fresh-frozen, (2) direct-fixed in formalin for 18 hours, (3) frozen then formalin-fixed, or (4) frozen then ethanol-fixed and paraffin-embedded (n=6/group/condition) tissue samples from the livers of mice exposed to 600 ppm phenobarbital vs. vehicle control for 7 days in drinking water. RNA sequencing data from paired fresh frozen and 18 hr. formalin-fixed paraffin-embedded liver tissue from mice administered 1500, 3000, and 6000 ppm DEHP or vehicle control for 7 days by dietary ingestion; or RNA sequencing data from paired fresh frozen, 18 hr. formalin fixed paraffin embedded, or 3 wk. formalin fixed paraffin embedded liver tissue from mice administered 8 mg/kg/d Furan or vehicle control for 3 weeks by gavage. This dataset is associated with the following publication: Wehmas, L., S. Hester, and C.E. Wood. Direct Formalin Fixation Induces Widespread Transcriptomic Effects in Archival Tissue Samples. Scientific Reports. Nature Publishing Group, London, UK, 10: 14497, (2020).

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  • Formalin_effect_MS_Master_Table_20200728_complete.xlsx

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  • Formalin_effect_MS_Master_Table_2_20200728.xlsx

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  • Formalin_effect_MS_Master_Table_3_20200728.xlsx

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  • Formalin_effect_MS_Master_Table_4_20200728.xlsx

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  • Formalin_effects_manuscript_supplementary_table_legends.docx

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Complete Metadata
@type dcat:Dataset
accessLevel public
bureauCode 
[
  "020:00"
]
contactPoint 
{
  "fn": "Leah Wehmas",
  "hasEmail": "mailto:wehmas.leah@epa.gov"
}
description RNA sequencing data from paired: (1) fresh-frozen, (2) direct-fixed in formalin for 18 hours, (3) frozen then formalin-fixed, or (4) frozen then ethanol-fixed and paraffin-embedded (n=6/group/condition) tissue samples from the livers of mice exposed to 600 ppm phenobarbital vs. vehicle control for 7 days in drinking water. RNA sequencing data from paired fresh frozen and 18 hr. formalin-fixed paraffin-embedded liver tissue from mice administered 1500, 3000, and 6000 ppm DEHP or vehicle control for 7 days by dietary ingestion; or RNA sequencing data from paired fresh frozen, 18 hr. formalin fixed paraffin embedded, or 3 wk. formalin fixed paraffin embedded liver tissue from mice administered 8 mg/kg/d Furan or vehicle control for 3 weeks by gavage. This dataset is associated with the following publication: Wehmas, L., S. Hester, and C.E. Wood. Direct Formalin Fixation Induces Widespread Transcriptomic Effects in Archival Tissue Samples. Scientific Reports. Nature Publishing Group, London, UK, 10: 14497, (2020).
distribution 
[
  {
    "title": "Formalin_effect_MS_Master_Table_20200728_complete.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1518503/Formalin_effect_MS_Master_Table_20200728_complete.xlsx"
  },
  {
    "title": "Formalin_effect_MS_Master_Table_2_20200728.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1518503/Formalin_effect_MS_Master_Table_2_20200728.xlsx"
  },
  {
    "title": "Formalin_effect_MS_Master_Table_3_20200728.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1518503/Formalin_effect_MS_Master_Table_3_20200728.xlsx"
  },
  {
    "title": "Formalin_effect_MS_Master_Table_4_20200728.xlsx",
    "mediaType": "application/vnd.openxmlformats-officedocument.spreadsheetml.sheet",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1518503/Formalin_effect_MS_Master_Table_4_20200728.xlsx"
  },
  {
    "title": "Formalin_effects_manuscript_supplementary_table_legends.docx",
    "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/1518503/Formalin_effects_manuscript_supplementary_table_legends.docx"
  }
]
identifier https://doi.org/10.23719/1518503
keyword 
[
  "DEHP",
  "Formalin-fixed paraffin embedded samples",
  "Gene expression profiles",
  "RNA-sequencing",
  "furan",
  "mode of action (MOA)",
  "molecular pathway analysis",
  "phenobarbital"
]
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2020-04-21
programCode 
[
  "020:095"
]
publisher 
{
  "name": "U.S. EPA Office of Research and Development (ORD)",
  "subOrganizationOf": {
    "name": "U.S. Environmental Protection Agency",
    "subOrganizationOf": {
      "name": "U.S. Government"
    }
  }
}
references 
[
  "https://doi.org/10.1038/s41598-020-71521-w"
]
rights 
null
title formalin effects data

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