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Chemical Analysis Method for Monitoring Metabolism in Live Cells: Data, Experiment Parameters, and SOPs

Published by U.S. Environmental Protection Agency | U.S. Environmental Protection Agency | Catalog Last Checked: September 10, 2026 at 07:03 PM | Dataset Last Updated: April 01, 2025
Chemical analysis method for real-time monitoring of metabolism and chemical uptake in live cells to reduce use of live animals in toxicity testing. Includes key data acquisition parameters, standardized procedures for samples preparation and data analysis (i.e., SOPs), and raw data files. This dataset is associated with the following publication: Ekman, D., and Q. Teng. Real-Time Untargeted Monitoring of Intracellular Metabolism in Live Cells Using 1H NMR and Extracellular Signal Suppression. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. American Chemical Society, Washington, DC, USA, 147(42): 38254-38264, (2025).

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Complete Metadata
accessLevel public
bureauCode 
[
  "020:00"
]
contactPoint 
{
  "fn": "Drew Ekman",
  "hasEmail": "mailto:ekman.drew@epa.gov"
}
description Chemical analysis method for real-time monitoring of metabolism and chemical uptake in live cells to reduce use of live animals in toxicity testing. Includes key data acquisition parameters, standardized procedures for samples preparation and data analysis (i.e., SOPs), and raw data files. This dataset is associated with the following publication: Ekman, D., and Q. Teng. Real-Time Untargeted Monitoring of Intracellular Metabolism in Live Cells Using 1H NMR and Extracellular Signal Suppression. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. American Chemical Society, Washington, DC, USA, 147(42): 38254-38264, (2025).
distribution 
[
  {
    "title": "JACS_Bruker Pulse Sequences.zip",
    "mediaType": "application/zip",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/d-2v76/JACS_Bruker%20Pulse%20Sequences.zip"
  },
  {
    "title": "JACS_Data Dictionary.docx",
    "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/d-2v76/JACS_Data%20Dictionary.docx"
  },
  {
    "title": "JACS_Data for Figures.zip",
    "mediaType": "application/zip",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/d-2v76/JACS_Data%20for%20Figures.zip"
  },
  {
    "title": "JACS_ExSS-NMR SOPs.zip",
    "mediaType": "application/zip",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/d-2v76/JACS_ExSS-NMR%20SOPs.zip"
  },
  {
    "title": "Readme.docx",
    "mediaType": "application/vnd.openxmlformats-officedocument.wordprocessingml.document",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/d-2v76/Readme.docx"
  },
  {
    "title": "topspin pulse-programming.pdf",
    "mediaType": "application/pdf",
    "downloadURL": "https://pasteur.epa.gov/uploads/10.23719/d-2v76/topspin%20pulse-programming.pdf"
  },
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]
identifier https://doi.org/10.23719/d-2v76
keyword 
[
  "NMR spectroscopy",
  "New Approach Methods (NAMs)",
  "in vitro screening",
  "metabolomics"
]
license https://pasteur.epa.gov/license/sciencehub-license.html
modified 2025-04-01
programCode 
[
  "020:000"
]
publisher 
{
  "name": "U.S. Environmental Protection Agency",
  "subOrganizationOf": {
    "name": "U.S. Government"
  }
}
references 
[
  "https://doi.org/10.1021/jacs.5c10909"
]
rights 
null
title Chemical Analysis Method for Monitoring Metabolism in Live Cells: Data, Experiment Parameters, and SOPs

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